ENZYMATIC CONVERSION OF A REDUCED DIPHOSPHOPYRIDINE NUCLEOTIDE DERIVATIVE TO REDUCED DIPHOSPHOPYRIDINE NUCLEOTIDE
نویسندگان
چکیده
منابع مشابه
Enzymatic conversion of a reduced diphosphopyridine nucleotide derivative to reduced diphosphopyridine nucleotide.
DPNH-X was prepared as described previously (1) ; the barium salt was converted to the sodium salt before use, and solutions were stored at 20”. Concentrations of DPNH-X were calculated with an extinction coefficient of 13.5 X lo6 sq. cm. mole-’ at 290 rnM (1). Sodium salts of ADP, ATP, ITP, UTP, and crystalline disodium ATP were obtained from the Pabst Laboratories, Milwaukee, Wisconsin. Myoki...
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A continuous flow instrumental system of analysis, first described by Skeggs (l), was previously applied to the determination of phosphohexose isomerase activity and resulted in a method that was approximately eightfold as rapid as the manual procedure (2). The present paper is concerned with the application of this system of analysis to the determination of enzyme activities that utilize the d...
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It has been previously shown (l-3) that the nicotinamide moiety of diphosphopyridine nucleotide can be replaced by other pyridine bases without the loss of the coenzyme function. The observation that both the 3-acetylpyridine and pyridine-3aldehyde analogues of diphosphopyridine nucleotide function as coensymes with various dehydrogenases (3) indicated that the amide group on DPN was not essent...
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We have shown in a previous communication (1) that the enzyme triosephosphate dehydrogenase is rapidly inactivated in the presence of reduced diphosphopyridine nucleotide at physiological concentrations, temperature, and pH. Good stoichiometry was shown for the amount of enzyme inactivation, DPNH-X1 formation, and disappearance of sulfhydryl groups. It was then of particular interest to determi...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1956
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)65307-4